DYNAMICS OF PRO- AND ANTI-INFLAMMATORY CYTOKINES IN PATIENTS WITH GENERALIZED PERIODONTITIS ACCOMPANIED BY DIFFERENT REACTIVITY OF THE ORGANISM.

The aim of this research was to study the dynamics of pro- and anti-inflammatory cytokines in the blood and gingival fluid of patients with generalized periodontitis accompanied by different reactivity of the body after patch surgery. The study included 216 people aged 45 between 55 years with a diagnosis of generalized periodontitis were examined. Depending on the condition of reactivity of the body, the patients were divided into three groups: 1 - normoreaction; 2- hyperreaction; 3 - hyporeaction. After the initial therapy, the patch was performed according to the indications. Blood sampling was performed after surgery on days 1, 2, 4, 6 and 9. The content of pro- (IL-1β, IL-6, TNFα) and anti-inflammatory (IL-4) cytokines in blood and gingival fluid was defined by solid-phase enzyme-linked immunosorbent assay. The results have shown that the normal reactivity of the organism is characterized by an increase in proinflammatory cytokines IL-1β, IL-6, TNFα at the time of increasing necrosis of periodontal tissues, their decrease and increase in IL-4 with the appearance of markers of reparative processes in blood and gingival fluid. Hyperreactivity is characterized by a more pronounced early increase in pro- and anti-inflammatory cytokines of blood and gingival fluid (IL-1β, IL-6, TNFα, IL-4) with a subsequent decrease below the initial values during the development of young connective tissue. In the case of hyporeactivity we can observe a late and less pronounced increase in all the cytokines in the blood and gingival fluid. Correction of altered parameters in patients with generalized periodontitis accompanied by impaired (hyper- and hypo-) reactivity of the body with bringing them to the values which are typical for normoreactivity is considered to be a condition for optimizing wound healing after surgery and further stabilization of the periodontal tissues.