[The progress in expression of genes of the oligodendrocyte lineage-myelin basic protein in oral lichen planus].

OBJECTIVE

The aim of this study was to determine the expression of genes of the oligodendrocyte lineage-myelin basic protein (Golli-MBP) in peripheral blood mononuclear cell (PBMC) in oral lichen planus (OLP) and to further understand the pathogenesis of OLP.

METHODS

PBMC was obtained by density gradient centrifugation, and the expression of Golli-MBP in PBMC was investigated in erythematous/erosive OLP (20 cases), reticular OLP (16 cases) and normal controls (19 cases) using reverse transcription-polymerase chain reaction (RT-PCR) and Western blot methods.

RESULTS

RT-PCR results showed that Golli-MBP mRNA was overexpressed in erythematous/erosive and reticular OLP as compared with normal control group (P < 0.01). Western blot assay indicated that erythematous/erosive and reticular OLP patients had a higher expression level of Golli-MBP protein in PBMC than normal controls (P < 0.01). However, there were no significant differences between erythematous/erosive and reticular groups in Golli-MBP mRNA and protein expression (P > 0.05).

CONCLUSION

The data accumulated here strongly indicate that Golli-MBP was involved in the pathogenesis of OLP.