Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology · 2011
Altered expression of miR-21, miR-125b, and miR-203 indicates a role for these microRNAs in oral lichen planus.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Danielsson K, Wahlin Y B, Gu X, Boldrup L, Nylander K
Аннотация
BACKGROUND: Oral lichen planus (OLP), which is a chronic inflammatory disease of the oral mucosa with unknown etiology, affects about 2% of the population. MicroRNAs are small non-coding RNAs involved in normal processes such as development and differentiation as well as progression of human diseases. The aim of this study was to investigate the expression of miR-21, miR-125b, and miR-203 and to compare RNA levels of their potential targets, the tumor suppressor p53 and its relative p63, both known to be deregulated in OLP.
METHODS: In biopsies from 20 patients with OLP and 20 age- and sex-matched healthy controls, epithelium was laser dissected and analyzed for the expression of miR-21, miR-125b, miR-203, p53, and p63 using qRT/PCR.
RESULTS: Increased expression of miR-21 and miR-203, decreased expression of miR-125, and down-regulation of p53 and ΔNp63 RNA were seen in OLP compared to normal oral mucosa. When comparing microRNA expression to levels of p53 and p63 RNA, a significant negative correlation was seen between ΔNp63 and miR-203 and between miR-21 and p53, respectively.
CONCLUSION: Results indicate a role for the studied microRNAs in changes seen in OLP.
METHODS: In biopsies from 20 patients with OLP and 20 age- and sex-matched healthy controls, epithelium was laser dissected and analyzed for the expression of miR-21, miR-125b, miR-203, p53, and p63 using qRT/PCR.
RESULTS: Increased expression of miR-21 and miR-203, decreased expression of miR-125, and down-regulation of p53 and ΔNp63 RNA were seen in OLP compared to normal oral mucosa. When comparing microRNA expression to levels of p53 and p63 RNA, a significant negative correlation was seen between ΔNp63 and miR-203 and between miR-21 and p53, respectively.
CONCLUSION: Results indicate a role for the studied microRNAs in changes seen in OLP.
Предметные рубрики MeSH
AdultAgedAged, 80 and overApoptosis Regulatory ProteinsBiopsyCase-Control StudiesDown-RegulationFemaleGene Expression RegulationHumansLichen Planus, OralMaleMicroRNAsMiddle AgedMouth MucosaReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionTranscription FactorsTumor Suppressor Protein p53Tumor Suppressor Proteins