FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016
A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.
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Makino Asami, Abe Mitsuhiro, Ishitsuka Reiko, Murate Motohide, Kishimoto Takuma, Sakai Shota, Hullin-Matsuda Françoise, Shimada Yukiko, Inaba Takehiko, Miyatake Hideyuki, Tanaka Hideko, Kurahashi Atsushi, Pack Chan-Gi, Kasai Rinshi S, Kubo Shuku, Schieber Nicole L, Dohmae Naoshi, Tochio Naoya, Hagiwara Kyoji, Sasaki Yutaka, Aida Yoko, Fujimori Fumihiro, Kigawa Takanori, Nishibori Kozo, Parton Robert G, Kusumi Akihiro, Sako Yasushi, Anderluh Gregor, Yamashita Makoto, Kobayashi Tetsuyuki, Greimel Peter, Kobayashi Toshihide
Аннотация
We identified a novel, nontoxic mushroom protein that specifically binds to a complex of sphingomyelin (SM), a major sphingolipid in mammalian cells, and cholesterol (Chol). The purified protein, termed nakanori, labeled cell surface domains in an SM- and Chol-dependent manner and decorated specific lipid domains that colocalized with inner leaflet small GTPase H-Ras, but not K-Ras. The use of nakanori as a lipid-domain-specific probe revealed altered distribution and dynamics of SM/Chol on the cell surface of Niemann-Pick type C fibroblasts, possibly explaining some of the disease phenotype. In addition, that nakanori treatment of epithelial cells after influenza virus infection potently inhibited virus release demonstrates the therapeutic value of targeting specific lipid domains for anti-viral treatment.-Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.-G., Kasai, R. S., Kubo, S., Schieber, N. L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton, R. G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T. A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.