Traditional Fermented Multi-Grain Supernatant (Ogi-baba Omidun) Restores Hypothalamic-Pituitary-Testicular Axis Function in a Juvenile Double-Hit PTSD-Neuroinflammation Rat Model.

Juvenile post-traumatic stress disorder (PTSD) compounded by systemic neuroinflammation poses a significant threat to neuroendocrine and reproductive development. This study evaluated the therapeutic potential of a traditional fermented multi-grain supernatant (Ogi-baba Omidun; MGS) and its postbiotics (PSB) in a juvenile rat model of PTSD-neuroinflammation comorbidity induced by single prolonged stress combined with lipopolysaccharide exposure. MGS and PSB significantly ameliorated anxiety-like behaviour and partially restored sexual performance, testicular testosterone, and luteinising hormone levels. Both preparations reduced oxidative stress markers and pro-inflammatory NF-κB signalling and increased anti-inflammatory IL-10 across the hypothalamic-pituitary-testicular axis. Histological and immunofluorescence analyses revealed partial preservation of neuroendocrine cytoarchitecture and attenuation of LC3B-II-associated autophagosome accumulation. These findings indicate that Ogi-baba Omidun exerts multi-target protective effects against the combined impact of traumatic stress and immune activation on the juvenile reproductive axis, supporting its translational potential as an accessible gut-mediated intervention.