Expression of extracellular markers as criteria for treatment of peripheral arterial occlusive disease.

UNLABELLED: During the last decade, mesenchymal stromal cells (MSC) have been used in medical therapeutics, especially for reperfusion in peripheral arterial disease, ischemic cardiomiopathy and myocardial infarction. Studies demonstrated that membrane proteins are important in MSC endothelial differentiation.

AIM: To determine the expression of extracellular markers of MSC, in order to ascertain if some expressed proteins (MSC specific cluster of differentiation proteins, essential for MSC characterization and proteins which have a role in adhesion and migration) have capability to induce angiogenesis.

MATERIAL AND

METHODS

To detect the expression of these proteins and confirm the presence of MSC and other essential proteins with important role in adhesion and migration, cells from five donors were fully assessed by flow cytometry (surface expression), differentiation assays (MSC differentiation into osteocytes, adipocytes, and chondrocytes), and qRT-PCR (gene relative expression).

RESULTS

Our results were in agreement with other reports, with small exceptions, related especially to donor dependent conditions. The surface expression of beta 1 subunits correlated with a high gene expression on RNA level.

CONCLUSIONS

MSC had all specific characteristics. Surface marker expression was in agreement with previous findings. These cells can be used for endothelial progenitor cells further differentiation. Beta 1 expression suggests a high potential of the MSC to form capillary lumen, thus for further artery formation and efficient angiogenesis.