Antigen-induced lymphocyte blastogenesis in a hookworm (Necator americanus) infection in man.

A lymphocyte blastogenic technique was employed to examine cell-mediated immunity in a Necator americanus infection. Infective third-stage larvae of N. americanus were jsed for antigen preparation, and lymphocyte cultures were prepared from peripheral blood taken on days 3, 17, and 51 after infection with 250 third-stage larvae of N. americanus. At 3 days post-infection lymphocyte transformation was not induced; at 17 and 51 days after infection significant transformation was obtained.