PloS one · 2019
Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China.
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Dan Jiaming, Zhang Xueping, Ren Zhihua, Wang Liqin, Cao Suizhong, Shen Liuhong, Deng Junliang, Zuo Zhicai, Yu Shumin, Wang Ya, Ma Xiaoping, Liu Haifeng, Zhou Ziyao, Hu Yanchun, Fu Hualin, He Changliang, Geng Yi, Gu Xiaobin, Peng Guangneng, Zhong Zhijun
Аннотация
Giardia duodenalis is a zoonotic parasitic protist and poses a threat to human and animal health. This study investigated the occurrence of G. duodenalis infection in post-weaned calves from Sichuan province, China. Faecal samples were collected from a total of 306 post-weaned calves (3-12 months old) from 10 farms, including 4 intensive feeding farms and 6 free-ranging farms. The overall infection rate of G. duodenalis was 41.2% (126/306) based on the PCR results at any of the three genetic loci: beta-giardin (bg), triose-phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. Giardia duodenalis assemblages E (n = 115, 91.3%), A (n = 3, 2.4%), and A mixed with E (n = 8, 6.3%) were identified among the 126 positive specimens. Multilocus sequence typing of G. duodenalis revealed 34 assemblage E multilocus genotypes (MLGs), 1 assemblage A MLG and 7 mixed assemblage (A and E) MLGs. The eBURST data showed a high degree of genetic diversity within assemblage E MLGs. The phylogenetic tree revealed that MLG E3 was the primary MLG subtype in Sichuan province and also the most widely distributed in China.