Current microbiology · 2011
Expression of ESBL-like activity in infrequently encountered members of the family Enterobacteriaceae.
Оценочное исследованиеСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Abbott Sharon L, Lidgard Janice A, Cheung Wendy K W, Obeso Martha N, Berrada Zenda L, Janda J Michael
Аннотация
A collection of 94 unusual members of the Enterobacteriaceae were screened for the presence of extended spectrum β-lactamases (ESBLs) using the MicroScan ESβL plus dried confirmation panel. Presumptively positive strains were then confirmed for the presence of an ESBL by double disk diffusion, E-test strips (AB Biodisk, Solna, Sweden) and PCR for SHV, TEM, and CTX-M2 genes. Of the 18 strains initially positive on the ESβL panel only three strains (Leminorella grimontii, Klebsiella ozaenae, and Kluyvera ascorbata) were positive by confirmation methods. These results suggest laboratories should be cautious regarding the methodology employed in screening for the presence of ESBLs in enteric bacteria. However, it should be noted that of the 94 strains, 29 were found to be resistant to two or more of the antibiotics present in the MicroScan ESβL plus panel indicating that there are potential treatment issues with these organisms despite their lack of ESBLs.