Journal of clinical pathology · 1997
Serological response of patients infected with Salmonella typhi.
Клиническое исследованиеСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Chart H, Rowe B, Cheesbrough J S
Аннотация
AIMS: To evaluate a rapid immunoblotting procedure for providing evidence of infection with Salmonella typhi using 73 sera from patients infected with S typhi.
METHODS: A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O = 9,12) and flagellar (H = d) antigens was used.
RESULTS: Seventy two of 73 sera contained antibodies to LPS, 40 sera also contained antibodies to H = d flagellar antigens. Analysis of acute and convalescent sera showed that only 62% of patients produced antibodies to flagellar antigens.
CONCLUSIONS: The SDS-PAGE/immunoblotting procedure provided a rapid method for providing serological evidence of infection with S typhi.
METHODS: A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O = 9,12) and flagellar (H = d) antigens was used.
RESULTS: Seventy two of 73 sera contained antibodies to LPS, 40 sera also contained antibodies to H = d flagellar antigens. Analysis of acute and convalescent sera showed that only 62% of patients produced antibodies to flagellar antigens.
CONCLUSIONS: The SDS-PAGE/immunoblotting procedure provided a rapid method for providing serological evidence of infection with S typhi.