Experimental eye research · 2026
Middle-wavelength green ambient light attenuates lens-induced myopia progression and is associated with suppression of the Wnt/β-catenin signaling pathway in guinea pigs.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Wu Haotian, Dong Li, Zhang Ruiheng, Shi Xuhan, Zhou Wenda, Li Heyan, Li Yitong, Yu Chuyao, Jonas Jost B, Wei Wenbin
Аннотация
PURPOSE: To investigate whether monochromatic ambient light modulates lens-induced myopia (LIM) progression in guinea pigs and whether these effects are associated with changes in Wnt/β-catenin signaling in the retina-choroid complex.
METHODS: Forty 3-week-old male pigmented guinea pigs were randomly assigned to four groups (n = 10/group): control, broadband white light with LIM (BW-LIM), middle-wavelength green light with LIM (MW-LIM), and short-wavelength blue light with LIM (SW-LIM). Bilateral LIM was induced using -10.0 D lenses for 4 weeks. Axial length and refractive error were measured weekly. Ocular structural changes were evaluated by fundus photography, optical coherence tomography, histology, and TUNEL staining. Expression of Wnt2b, Fzd5, and β-catenin in the retina-choroid complex was assessed by RT-PCR and Western blot.
RESULTS: After 4 weeks, compared with the control group, the BW-LIM group showed an axial elongation of 0.25 ± 0.02 mm and a myopic shift of -4.9 ± 0.3 D (both P < 0.0001). Relative to the BW-LIM group, the MW-LIM group showed significantly less axial elongation (0.16 ± 0.02 mm vs. 0.25 ± 0.02 mm, P = 0.025) and a smaller myopic shift (-3.75 ± 0.3 D vs. -4.9 ± 0.3 D, P = 0.005), whereas the SW-LIM group did not differ significantly from the BW-LIM group. Reduced axial elongation under middle-wavelength light was mainly attributable to less vitreous chamber deepening (difference vs. BW-LIM: 0.10 ± 0.03 mm, P = 0.03). All LIM groups showed choroidal and scleral thinning, which was attenuated in the MW-LIM group but not in the SW-LIM group. No overt retinal structural damage or increase in apoptosis was detected under either monochromatic lighting condition. At the transcriptional level, Wnt2b, Fzd5, and β-catenin were significantly upregulated in the BW-LIM group versus controls. In the MW-LIM group, expression levels were lower than those in the BW-LIM group, whereas the SW-LIM group showed no significant difference from the BW-LIM group. Protein expression showed a similar pattern.
CONCLUSIONS: Ambient middle-wavelength green light attenuated vitreous chamber deepening and slowed LIM progression in guinea pigs and was associated with downregulation of Wnt/β-catenin signaling in the retina-choroid complex. Short-wavelength light showed no significant effect compared with broadband-light LIM.
METHODS: Forty 3-week-old male pigmented guinea pigs were randomly assigned to four groups (n = 10/group): control, broadband white light with LIM (BW-LIM), middle-wavelength green light with LIM (MW-LIM), and short-wavelength blue light with LIM (SW-LIM). Bilateral LIM was induced using -10.0 D lenses for 4 weeks. Axial length and refractive error were measured weekly. Ocular structural changes were evaluated by fundus photography, optical coherence tomography, histology, and TUNEL staining. Expression of Wnt2b, Fzd5, and β-catenin in the retina-choroid complex was assessed by RT-PCR and Western blot.
RESULTS: After 4 weeks, compared with the control group, the BW-LIM group showed an axial elongation of 0.25 ± 0.02 mm and a myopic shift of -4.9 ± 0.3 D (both P < 0.0001). Relative to the BW-LIM group, the MW-LIM group showed significantly less axial elongation (0.16 ± 0.02 mm vs. 0.25 ± 0.02 mm, P = 0.025) and a smaller myopic shift (-3.75 ± 0.3 D vs. -4.9 ± 0.3 D, P = 0.005), whereas the SW-LIM group did not differ significantly from the BW-LIM group. Reduced axial elongation under middle-wavelength light was mainly attributable to less vitreous chamber deepening (difference vs. BW-LIM: 0.10 ± 0.03 mm, P = 0.03). All LIM groups showed choroidal and scleral thinning, which was attenuated in the MW-LIM group but not in the SW-LIM group. No overt retinal structural damage or increase in apoptosis was detected under either monochromatic lighting condition. At the transcriptional level, Wnt2b, Fzd5, and β-catenin were significantly upregulated in the BW-LIM group versus controls. In the MW-LIM group, expression levels were lower than those in the BW-LIM group, whereas the SW-LIM group showed no significant difference from the BW-LIM group. Protein expression showed a similar pattern.
CONCLUSIONS: Ambient middle-wavelength green light attenuated vitreous chamber deepening and slowed LIM progression in guinea pigs and was associated with downregulation of Wnt/β-catenin signaling in the retina-choroid complex. Short-wavelength light showed no significant effect compared with broadband-light LIM.