Clinical laboratory · 2022
IncRNA XIST Targets miR-124/JAG1 via CeRNA Mechanism to Facilitate the Migration and Proliferation of Tongue Squamous Cell Carcinoma.
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Gao Shuichao, Yu Jianjun, Shan Zhenfeng, Zuo Liang, Huang Weilun, Gan Li, Tian Hao
Аннотация
BACKGROUND: This study was designed to characterize the effect of lncRNA XIST on the migration as well as proliferation of tongue squamous cell carcinoma (TSCC) and its associated molecular mechanisms.
METHODS: Chip-seq analysis screened the aberrant lncRNA in TSCC patients. CCK-8 and scratch experiment detected cell migration and proliferation in TSCC after lncRNA XIST inhibition. We predicted and verified lncRNA XIST target miRNA. CCK-8 and scratch test examined the cell migration and proliferation effects in TSCC after transfection of miR-124 mimics. Luciferase reporter experiment confirmed the interaction of miR-124 with JAG1. Western blot validated influence of lncRNA XIST on miR-124/JAG1 axis.
RESULTS: We found that lncRNA XIST was up-regulated in TSCC patients, and it facilitated the TSCC cell migration and proliferation in vitro. lncRNA XIST regulated miR-124 expression through ceRNA mechanism. Up-regulating miR-124 significantly inhibited TSCC cell migration and proliferation. JAG1 acted as immediate target of miR-124. Moreover, lncRNA XIST targeted miR-124 to regulate JAG1 levels through the ceRNA mechanism.
CONCLUSIONS: IncRNA XIST encourages TSCC migration and proliferation by modulating miR-124/JAG1 axis.
METHODS: Chip-seq analysis screened the aberrant lncRNA in TSCC patients. CCK-8 and scratch experiment detected cell migration and proliferation in TSCC after lncRNA XIST inhibition. We predicted and verified lncRNA XIST target miRNA. CCK-8 and scratch test examined the cell migration and proliferation effects in TSCC after transfection of miR-124 mimics. Luciferase reporter experiment confirmed the interaction of miR-124 with JAG1. Western blot validated influence of lncRNA XIST on miR-124/JAG1 axis.
RESULTS: We found that lncRNA XIST was up-regulated in TSCC patients, and it facilitated the TSCC cell migration and proliferation in vitro. lncRNA XIST regulated miR-124 expression through ceRNA mechanism. Up-regulating miR-124 significantly inhibited TSCC cell migration and proliferation. JAG1 acted as immediate target of miR-124. Moreover, lncRNA XIST targeted miR-124 to regulate JAG1 levels through the ceRNA mechanism.
CONCLUSIONS: IncRNA XIST encourages TSCC migration and proliferation by modulating miR-124/JAG1 axis.