Медицинская библиотека · Antiviral therapy · 2019

HSV susceptibility to acyclovir - genotypic and phenotypic characterization.

Vikas Raksha, Prabhu Suresha G, Mudgal Piya P, Shetty Ujwal, Karunakaran Kavitha, Jagadesh Anitha, Auti Amogh, Stansilaus Rithu P, Nair Sudheesh, Arunkumar Govindakarnavar

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Аннотация

BACKGROUND

Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples.

METHODS

A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique.

RESULTS

Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay.

CONCLUSIONS

These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.

Для цитирования: Vikas Raksha, Prabhu Suresha G, Mudgal Piya P, Shetty Ujwal, Karunakaran Kavitha, Jagadesh Anitha, Auti Amogh, Stansilaus Rithu P, Nair Sudheesh, Arunkumar Govindakarnavar. HSV susceptibility to acyclovir - genotypic and phenotypic characterization.. Antiviral therapy. 2019. DOI: 10.3851/imp3279.

Предметные рубрики MeSH

Acyclovir Adult Antiviral Agents Drug Resistance, Viral Female Genes, Viral Genotype Herpes Simplex Herpesvirus 1, Human Herpesvirus 2, Human Humans Male Microbial Sensitivity Tests Mutation Phenotype Retrospective Studies Sequence Analysis, DNA Young Adult

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