Microbial pathogenesis · 2023
The impact of ORF19.36.1 in the pathobiology of Candida albicans.
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Arita Glaucia Sayuri, Ma Qinxi, Leaves Ian, Pradhan Arnab, Hickey Emer, Dambuza Ivy, Bebes Attila, Vincenzi Conrado Pollyanna Cristina, Barros Galinari Camila, Vicente Seixas Flávio Augusto, Kioshima Érika Seki, de Souza Bonfim-Mendonça Patrícia, Svidzinski Terezinha Inez Estivalet, Brown Alistair J P
Аннотация
BACKGROUND: Our previous proteomics data obtained from Candida albicans recovered after serial passage in a murine model of systemic infection revealed that Orf19.36.1 expression correlates with the virulence of the fungus. Therefore, the impact of ORF19.36.1 upon virulence was tested in this study.
MATERIALS & METHODS: CRISPR-Cas9 technology was used to construct homozygous C. albicans orf19.36.1 null mutants and the phenotypes of these mutants examined in vitro (filamentation, invasion, adhesion, biofilm formation, hydrolase activities) and in vivo assays.
RESULTS: The deletion of ORF19.36.1 did not significantly impact the phenotypes examined or the virulence of C. albicans in two infection models.
CONCLUSION: These results suggest that, although Orf19.36.1 expression correlates with virulence, this protein is not essential for C. albicans pathobiology.
MATERIALS & METHODS: CRISPR-Cas9 technology was used to construct homozygous C. albicans orf19.36.1 null mutants and the phenotypes of these mutants examined in vitro (filamentation, invasion, adhesion, biofilm formation, hydrolase activities) and in vivo assays.
RESULTS: The deletion of ORF19.36.1 did not significantly impact the phenotypes examined or the virulence of C. albicans in two infection models.
CONCLUSION: These results suggest that, although Orf19.36.1 expression correlates with virulence, this protein is not essential for C. albicans pathobiology.