Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University · 2002
[mRNA expression of transforming growth factor-beta 1 in the cornea after photorefractive keratectomy in rabbits].
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Xu Hui-zhuo, Liu Shuang-zhen, Xia Xiao-bo
Аннотация
OBJECTIVE: To investigate the roles of transforming growth factor beta 1 (TGF-beta 1) during healing after photorefractive keratectomy (PRK).
METHODS: Twenty of 24 rabbits underwent bilateral 193 nm excimer laser photorefractive keratectomy to correct 10 diopters of myopia, and the other 4 rabbits were taken as the normal group. The mRNA levels of TGF-beta 1 in the corneas of the rabbits were determined by in situ hybridization with a Digoxigenin labled probe.
RESULTS: 1. Corneal haze appeared in operated eyes from the 7th day, and peaked on the 28th day after PRK. The most severe haze reached Grade 3. 2. In the normal group, a low level of mRNA expression of TGF-beta 1 was observed in the epithelium, and no positive result in the stroma. On the 7th, 14th, and 28th days after the operation, the mRNA levels of TGF-beta 1 in the epithelium and stroma of the operation group were significantly higher than those in the normal group.
CONCLUSION: TGF-beta 1 may involve in corneal wound healing after PRK and may increase haze by promoting the synthesis of the extracellular matrix and the proliferation of keratocytes.
METHODS: Twenty of 24 rabbits underwent bilateral 193 nm excimer laser photorefractive keratectomy to correct 10 diopters of myopia, and the other 4 rabbits were taken as the normal group. The mRNA levels of TGF-beta 1 in the corneas of the rabbits were determined by in situ hybridization with a Digoxigenin labled probe.
RESULTS: 1. Corneal haze appeared in operated eyes from the 7th day, and peaked on the 28th day after PRK. The most severe haze reached Grade 3. 2. In the normal group, a low level of mRNA expression of TGF-beta 1 was observed in the epithelium, and no positive result in the stroma. On the 7th, 14th, and 28th days after the operation, the mRNA levels of TGF-beta 1 in the epithelium and stroma of the operation group were significantly higher than those in the normal group.
CONCLUSION: TGF-beta 1 may involve in corneal wound healing after PRK and may increase haze by promoting the synthesis of the extracellular matrix and the proliferation of keratocytes.