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DOI 10.2478/s11686-020-00194-2

Медицинская библиотека · Acta parasitologica · 2020

Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil.

Dos Santos Silva Ana Clécia, de Barros Luiz Daniel, Barros Viviane Melo Coelho, de Alcântara Adrianne Mota, Andrade Müller Ribeiro, Garcia João Luis, Mota Rinaldo Aparecido, Porto Wagnner José Nascimento

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Аннотация

PURPOSE: The aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil.

METHODS

Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brain tissue from 14 animals randomly selected were subjected to mouse bioassay. Positive samples in mouse bioassay were submitted to PCR and genotyped by PCR-RFLP.

RESULTS

Out of two hundred blood samples from chickens, 72 (36%) samples were considered positive by IFAT. Two T. gondii strains were isolated, both being characterized as atypical and classified as #146 and a new genotype, named #279 in ToxoDB database.

CONCLUSIONS

Our results showed a sero-occurrence of T. gondii in free-range chickens intended for humans, and the genetic diversity of the parasite in Brazil, with a new genotype described.

Для цитирования: Dos Santos Silva Ana Clécia, de Barros Luiz Daniel, Barros Viviane Melo Coelho, de Alcântara Adrianne Mota, Andrade Müller Ribeiro, Garcia João Luis, Mota Rinaldo Aparecido, Porto Wagnner José Nascimento. Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil.. Acta parasitologica. 2020. DOI: 10.2478/s11686-020-00194-2.

Предметные рубрики MeSH

AnimalsAntibodies, ProtozoanBiological AssayBrazilChickensDNA, ProtozoanFood MicrobiologyGenetic VariationGenotypeHumansMicePoultryPoultry DiseasesToxoplasmaToxoplasmosis, Animal
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