Molecular detection of Toxoplasma gondii from aborted fetuses of sheep, goats and cattle in Bangladesh.

The study was planned to apply the PCR method for detection of T. gondii infection in sheep, goats and cattle aborted fetuses from Mymensingh, Bangladesh. A total of 58 fetal tissue samples (brain, liver, heart, skeletal muscle and placenta) of sheep (5), goats (5) and cattle (2) were selected for study. Aborted fetuses were taken from serologically positive mothers by indirect ELISA. Among them 24 and 34 samples were subjected for PCR assay by using TgB1 and TgTox4 primers respectively. DNA fragments were visualized under UV illumination after gel run. The results demonstrated 15.52% tissue samples from sheep and goat aborted fetuses were positive for T. gondii parasite. Among different tissue samples, brain, liver and heart showed presence of T. gondii parasite. None of tissue samples showed positive in case of cattle. The results of the PCR exhibited that T. gondii infection might be considered as one of the major causative agents for abortion in ewes and does. Further studies are needed to improve our knowledge on different genotypes of T. gondii that infect sheep, goat and cattle population in Bangladesh.