Zhonghua fu chan ke za zhi · 1997
[The secretion and receptor gene of insulin-like growth factor-I quantitative expression in ovarian stroma in polycystic ovarian syndrome patients].
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Liang X, Zhuang G, Fang Q
Аннотация
OBJECTIVE: To explore varying different degrees of the action of insulin-like growth factor-I (IGF-I) and its receptor gene quantitative expression in the ovarian stroma of women with obese and nonobese polycystic ovarian syndrome (PCOS).
METHOD: The expression of IGF-I receptor gene in the ovarian stroma from 35 cases of PCOS (obese: 15 cases; nonobese: 20 cases) and 20 cases of control were quantitatively determined by reverse transcript polymerase chain reaction (RT-PCR) combined with beta(2) micro globlin (beta(2) MG) gene as inner-control to amplificate sychronously. The grey value ratio of them were determined. The PCR-amplified cDNA fragments were put in restriction analysis (DdeI).
RESULTS: The expression of IGF-I receptor in PCOS ovary stroma was significantly greater than in the control group (P < 0.001). The nonobese group presents more enhancement than obese group in IGF-I receptor gene expression. The restrictive fragments were same between the PCOS and the control group.
CONCLUSIONS: The expression of IGF-I receptor in PCOS ovarian stroma is abnormally excessive and the nonobese PCOS have more potential action of IGF-I system in ovary stroma.
METHOD: The expression of IGF-I receptor gene in the ovarian stroma from 35 cases of PCOS (obese: 15 cases; nonobese: 20 cases) and 20 cases of control were quantitatively determined by reverse transcript polymerase chain reaction (RT-PCR) combined with beta(2) micro globlin (beta(2) MG) gene as inner-control to amplificate sychronously. The grey value ratio of them were determined. The PCR-amplified cDNA fragments were put in restriction analysis (DdeI).
RESULTS: The expression of IGF-I receptor in PCOS ovary stroma was significantly greater than in the control group (P < 0.001). The nonobese group presents more enhancement than obese group in IGF-I receptor gene expression. The restrictive fragments were same between the PCOS and the control group.
CONCLUSIONS: The expression of IGF-I receptor in PCOS ovarian stroma is abnormally excessive and the nonobese PCOS have more potential action of IGF-I system in ovary stroma.