Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2015
Sonic hedgehog signaling mediates resveratrol to increase proliferation of neural stem cells after oxygen-glucose deprivation/reoxygenation injury in vitro.
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Cheng Wei, Yu Pingping, Wang Li, Shen Changbo, Song Xiaosong, Chen Jixiang, Tang Fanren, Yang Qin
Аннотация
BACKGROUND/AIMS: There is interest in drugs and rehabilitation methods to enhance neurogenesis and improve neurological function after brain injury or degeneration. Resveratrol may enhance hippocampal neurogenesis and improve hippocampal atrophy in chronic fatigue mice and prenatally stressed rats. However, its effect and mechanism of neurogenesis after stroke is less well understood. Sonic hedgehog (Shh) signaling is crucial for neurogenesis in the embryonic and adult brain, but relatively little is known about the role of Shh signaling in resveratrol-enhanced neurogenesis after stroke.
METHODS: Neural stem cells (NSCs) before oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro were pretreated with resveratrol with or without cyclopamine. Survival and proliferation of NSCs was assessed by the CCK8 assay and BrdU immunocytochemical staining. The expressions and activity of signaling proteins and mRNAs were detected by immunocytochemistry, Western blotting, and RT-PCR analysis.
RESULTS: Resveratrol significantly increased NSCs survival and proliferation in a concentration-dependent manner after OGD/R injury in vitro. At the same time, the expression of Patched-1, Smoothened (Smo), and Gli-1 proteins and mRNAs was upregulated, and Gli-1 entered the nucleus, which was inhibited by cyclopamine, a Smo inhibitor.
CONCLUSION: Shh signaling mediates resveratrol to increase NSCs proliferation after OGD/R injury in vitro.
METHODS: Neural stem cells (NSCs) before oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro were pretreated with resveratrol with or without cyclopamine. Survival and proliferation of NSCs was assessed by the CCK8 assay and BrdU immunocytochemical staining. The expressions and activity of signaling proteins and mRNAs were detected by immunocytochemistry, Western blotting, and RT-PCR analysis.
RESULTS: Resveratrol significantly increased NSCs survival and proliferation in a concentration-dependent manner after OGD/R injury in vitro. At the same time, the expression of Patched-1, Smoothened (Smo), and Gli-1 proteins and mRNAs was upregulated, and Gli-1 entered the nucleus, which was inhibited by cyclopamine, a Smo inhibitor.
CONCLUSION: Shh signaling mediates resveratrol to increase NSCs proliferation after OGD/R injury in vitro.
Предметные рубрики MeSH
AnimalsAnti-Inflammatory Agents, Non-SteroidalCell ProliferationCell SurvivalFemaleGlucoseHedgehog ProteinsKruppel-Like Transcription FactorsMaleNestinNeural Stem CellsOxygenPatched ReceptorsPatched-1 ReceptorRatsRats, Sprague-DawleyReceptors, Cell SurfaceReceptors, G-Protein-CoupledResveratrolReverse Transcriptase Polymerase Chain ReactionSignal TransductionSmoothened ReceptorStilbenesVeratrum AlkaloidsZinc Finger Protein GLI1