Japanese journal of clinical oncology · 2009
No association of the NFKB1 insertion/deletion promoter polymorphism with survival in patients with gastric cancer.
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Kim Jong Gwang, Sohn Sang Kyun, Chae Yee Soo, Moon Joon Ho, Kim Shi Nae, Kang Byung Woog, Kim Gab Chul, Lee Myung-Hoon, Jeon Seong Woo, Chung Ho Young, Yu Wansik
Аннотация
OBJECTIVE: The present study analyzed the functional insertion/deletion polymorphism in the promoter region of NFKB1 gene and their impact on the prognosis for patients with gastric adenocarcinoma.
METHODS: Four hundred and seventy two consecutive patients with curatively resected gastric adenocarcinoma were enrolled in the present study. The genomic DNA was extracted from paraffin-embedded tissue and the -94 insertion/deletion ATTG polymorphism of NFKB1 determined using a polymerase chain reaction-restriction fragment length polymorphism assay.
RESULTS: The NFKB1 promoter gene polymorphism was successfully amplified in 97.8% of the cases. There were no sexual differences in relation to the genotype and allele. No correlation was observed between the frequency of the genotype or allele and the T, N or M stage. The multivariate survival analysis showed no association between the NFKB1 -94 insertion/deletion promoter polymorphism and the disease-free survival or overall survival of the patients with gastric cancer.
CONCLUSIONS: The functional NFKB1 promoter polymorphism was not found to be a prognostic marker for Korean patients with surgically resected gastric adenocarcinoma.
METHODS: Four hundred and seventy two consecutive patients with curatively resected gastric adenocarcinoma were enrolled in the present study. The genomic DNA was extracted from paraffin-embedded tissue and the -94 insertion/deletion ATTG polymorphism of NFKB1 determined using a polymerase chain reaction-restriction fragment length polymorphism assay.
RESULTS: The NFKB1 promoter gene polymorphism was successfully amplified in 97.8% of the cases. There were no sexual differences in relation to the genotype and allele. No correlation was observed between the frequency of the genotype or allele and the T, N or M stage. The multivariate survival analysis showed no association between the NFKB1 -94 insertion/deletion promoter polymorphism and the disease-free survival or overall survival of the patients with gastric cancer.
CONCLUSIONS: The functional NFKB1 promoter polymorphism was not found to be a prognostic marker for Korean patients with surgically resected gastric adenocarcinoma.
Предметные рубрики MeSH
AdenocarcinomaAdultAgedAged, 80 and overDNA, NeoplasmFeasibility StudiesFemaleGene DeletionHumansKoreaMaleMiddle AgedMutagenesis, InsertionalNF-kappa B p50 SubunitNeoplasm StagingPolymerase Chain ReactionPolymorphism, Single NucleotidePrognosisPromoter Regions, GeneticStomach NeoplasmsSurvival RateTreatment Outcome