[Effect of zibuganshenfang on the expression and activity of tyrosinase on B16 cells].

OBJECTIVE

ZiBuGanShenFang (ZBGSF) is a traditional herbal formula, which has showed an outstanding therapeutic effect on vitiligo clinically. Our aim is to investigate the influence of ZBGSF drug serum on the expression and activity of Tyrosinase in B16 cells, explore the mechanism of ZBGSF on Vitiligo treatment further.

METHOD: tyrosinase activity was measured by zymological methods, western blotting and RT-PCR were used to measure the protein content and mRNA level of tyrosinase and related proteins, respectively.

RESULT: ZBGSF drug serum had no effect on the proliferation of B16 cells. But it could promote Tyrosinase activity significantly and increase protein content and mRNA level of tyrosinase and related proteins in B16 cells.

CONCLUSION

Promoting the expression of tyrosinase protein and mRNA may be the elementary basis of ZBGSF on Vitiligo treatment.