Protective effect of osmanthus water extract on liver dysfunction caused by DBP based on organoids and organ chips technologies.

This study aimed to investigate the protective mechanism of Osmanthus fragrans water extract (OSF) against liver injury induced by dibutyl phthalate (DBP). We utilized liver organoids and liver organ chip technology to replicate the liver microenvironment in vivo. Metabolomic analysis revealed that DBP induced oxidative stress and lipid metabolism disorders; however, following intervention with OSF, the associated abnormal metabolites were significantly reduced. Molecular docking studies demonstrated the binding of its active ingredients to key targets. The primary findings indicate that OSF can effectively mitigate liver damage caused by DBP. This extract is anticipated to serve as a potential natural therapeutic agent for the prevention and treatment of DBP-induced liver dysfunction, thereby providing a theoretical foundation for future research and development.