Prenatal Smoking Exposures and Epigenome-Wide Methylation in Newborn Blood.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Hoang Thanh T, Cosin-Tomas Marta, Lee Yunsung, Monasso Giulietta, Xu Zongli, Li Sebastian Shaobo, Zeng Xuehuo, Starling Anne P, Reimann Brigitte, Röder Stefan, Zillich Lea, Jima Dereje D, Thio Chris H L, Pesce Giancarlo, Kersten Elin T G, Breeze Charles E, Burkholder Adam B, Lee Mikyeong, Ward James M, BIOS Consortium, Alfano Rossella, Deuschle Michael, Duijts Liesbeth, Ghassabian Akhgar, Herrera Laura-Concepció Gómez, Jaddoe Vincent Wv, Motsinger-Reif Alison A, Lie Rolv T, Nawrot Tim S, Page Christian M, Send Tabea S, Sharp Gemma, Stein Dan J, Streit Fabian, Sunyer Jordi, Wilcox Allen J, Zar Heather J, Koppelman Gerard H, Annesi-Maesano Isabella, Corpeleijn Eva, Snieder Harold, Hoyo Cathrine, Hüls Anke, Sirignano Lea, Witt Stephanie H, Herberth Gunda, Plusquin Michelle, Dabelea Dana, Yeung Edwina, Wiemels Joseph L, Richmond Rebecca C, Taylor Jack A, Felix Janine F, Håberg Siri E, Bustamante Mariona, London Stephanie J
Аннотация
OBJECTIVE: To identify differential DNA methylation in newborns from maternal sustained smoking and less studied prenatal smoking exposures (i.e., maternal exposure to secondhand smoke [SHS] exposure during pregnancy, maternal quitting before pregnancy, paternal smoking around conception, and paternal quitting before pregnancy).
METHODS: We conducted a large meta-analysis of prenatal tobacco smoking exposures and epigenome-wide newborn blood DNA methylation through the Pregnancy And Childhood Epigenetics Consortium (PACE). Across 19 cohorts, 11,175 parent-newborn pairs contributed information on at least one prenatal smoking exposure, mostly from questionnaires. Maternal blood or urine cotinine measurements, available in a few studies, provided objective data for maternal SHS and smoking during pregnancy. Primary analyses used Illumina450 K methylation data; secondary analyses in 5 cohorts examined CpGs unique to the EPIC array.
RESULTS: Maternal sustained smoking associated with differential DNA methylation (false discovery rate [FDR] < 0.05) at 8,862 CpGs on the 450 K (n = 8,148) and did not differ by infant sex. We identified over 300 novel genes not previously identified in EWAS of smoking. No differential methylation was associated with maternal SHS, maternal former smoking, or paternal smoking around conception. However, cg24805739 (MED13L) was associated with former paternal former smoking. Forty-one novel genes were identified using maternal cotinine measurements compared to questionnaire. In EPIC unique analyses (n = 3,415), differential methylation was observed with maternal sustained smoking (211 CpGs), maternal SHS (5 CpGs), and paternal former smoking (4 CpGs). Smoking-associated CpGs in blood were strongly enriched for functional elements across multiple tissues.
CONCLUSIONS: Maternal sustained smoking has the largest impact on newborn DNA methylation, suggesting a strong influence of the intrauterine environment. We observed minimal impacts for less studied exposures including SHS, maternal former smoking, and paternal smoking.