Photodiagnosis and photodynamic therapy · 2025
Ultrasonic chamber combined with photodynamic therapy inhibits bacterial growth on dental devices.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ribeiro Jeter Bochnia, Foggiato Augusto Alberto, Fernandes Douglas Silva, Câmara João Victor Frazão, Ribeiro Amanda de Oliveira Pinto, França Fabiana Mantovani Gomes
Аннотация
BACKGROUND: Photodynamic therapy is a two-stage treatment that combines light energy with a photosensitizer, and enhances the treatment against bacterial infections. In this context, the present study evaluated a newly patented device, called an ultrasonic photodynamic inactivation device (UPID), which performs microbial inactivation using photodynamic therapy for both prosthetic braces (PBs) and prototyped surgical guides (PSGs).
METHODS: Photodynamic inactivation was analyzed by contaminating the instruments with bacterial suspensions (3 × 108 CFU/mL) of 100 µM/L methylene blue solution for 20 min, followed by irradiation (0.30 J/cm2) with red light-emitting diode (660 nm) for 20 min, on three types of microorganisms: Candida albicans ATCC 10,231, Staphylococcus aureus ATCC 25,923 and Escherichia coli ATCC 25,922. The PSGs included a group with irradiation for 30 min (0.45 J/cm2), and a control group with 0.2 % peracetic acid, evaluated at both 20-minute time points. Microbial inhibition was assessed by counting the number of colony-forming units (CFU), and by the data evaluated using the Shapiro-Wilk, Mann-Whitney-U, and Kruskal-Wallis tests, at a 5 % significance level.
RESULTS: All experimental treatments showed significant reduction in log CFU/mL. The UPID promoted a significant microbial reduction (p < 0.001), compared with the positive control. In addition, peracetic acid was more effective than PDT for the PSG (p < 0.001). However, after 20 min, both treatments protected the surface material against bacterial growth.
CONCLUSION: The device proved effective for microbial inhibition of PB and PSG, thus proposing a new technique for the non-toxic disinfection of biomedical devices.
METHODS: Photodynamic inactivation was analyzed by contaminating the instruments with bacterial suspensions (3 × 108 CFU/mL) of 100 µM/L methylene blue solution for 20 min, followed by irradiation (0.30 J/cm2) with red light-emitting diode (660 nm) for 20 min, on three types of microorganisms: Candida albicans ATCC 10,231, Staphylococcus aureus ATCC 25,923 and Escherichia coli ATCC 25,922. The PSGs included a group with irradiation for 30 min (0.45 J/cm2), and a control group with 0.2 % peracetic acid, evaluated at both 20-minute time points. Microbial inhibition was assessed by counting the number of colony-forming units (CFU), and by the data evaluated using the Shapiro-Wilk, Mann-Whitney-U, and Kruskal-Wallis tests, at a 5 % significance level.
RESULTS: All experimental treatments showed significant reduction in log CFU/mL. The UPID promoted a significant microbial reduction (p < 0.001), compared with the positive control. In addition, peracetic acid was more effective than PDT for the PSG (p < 0.001). However, after 20 min, both treatments protected the surface material against bacterial growth.
CONCLUSION: The device proved effective for microbial inhibition of PB and PSG, thus proposing a new technique for the non-toxic disinfection of biomedical devices.