International journal of clinical pediatric dentistry · 2025
Exploring the Oral Microbiome in the Dental Biofilm of Children with Caries from Vulnerable Communities in Cartagena, Colombia: A Cross-sectional Study.
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Fang Luis, Herrera-Herrera Alejandra, Harris-Ricardo Jonathan, Olier-Castillo Doris, Fortich-Mesa Natalia, Hoyos-Hoyos Vivi
Аннотация
AIMS AND BACKGROUND: To explore the oral microbiome in the cariogenic dental biofilm in children from vulnerable communities in Cartagena, Colombia.
MATERIALS AND METHODS: We conducted a prevalence study that included 75 children aged 5-8 from three vulnerable communities in Cartagena, Colombia. Taking into account the International Caries Detection and Assessment System (ICDAS), calibrated examiners diagnosed all participants with dental caries. Samples of dental biofilm were obtained from carious tooth surfaces; extraction of bacterial DNA (deoxyribonucleic acid) was performed, and sequenced V3-V4 region of 16S rRNA (ribosomal ribonucleic acid) gene. Statistical analyses were conducted at the genus and species levels. Microbial diversity was analyzed using the Shannon diversity index.
RESULTS: The most prevalent bacterial genera identified were Streptococcus (probe 4), Lactobacillus (probe 5), Streptococcus (probe 1), and Fusobacterium (probe 4). The most abundant species identified were Lactobacillus salivarius, Streptococcus mutans, Streptococcus sanguinis, and Leptotrichia sp. HOT 417. A comparison of relative species abundance showed a more significant presence of Parascardovia denticolens, S. mutans, and Streptococcus sobrinus in extensive cavitated carious lesions (p < 0.001). The oral biofilm exhibited richness in bacterial species, as the α-Shannon index of 3.02 (SD 0.36) indicated.
CONCLUSION: The oral microbiome in the cariogenic dental biofilm in children from vulnerable communities in Cartagena shows high bacterial diversity, with specific species significantly associated with extensive cavitated lesions.
CLINICAL SIGNIFICANCE: Understanding the microbial diversity and specific bacterial associations in carious lesions provides valuable insights for developing targeted preventive and therapeutic strategies.
HOW TO CITE THIS ARTICLE: Fang L, Herrera-Herrera A, Harris-Ricardo J, et al. Exploring the Oral Microbiome in the Dental Biofilm of Children with Caries from Vulnerable Communities in Cartagena, Colombia: A Cross-sectional Study. Int J Clin Pediatr Dent 2025;18(10):1211-1216.
MATERIALS AND METHODS: We conducted a prevalence study that included 75 children aged 5-8 from three vulnerable communities in Cartagena, Colombia. Taking into account the International Caries Detection and Assessment System (ICDAS), calibrated examiners diagnosed all participants with dental caries. Samples of dental biofilm were obtained from carious tooth surfaces; extraction of bacterial DNA (deoxyribonucleic acid) was performed, and sequenced V3-V4 region of 16S rRNA (ribosomal ribonucleic acid) gene. Statistical analyses were conducted at the genus and species levels. Microbial diversity was analyzed using the Shannon diversity index.
RESULTS: The most prevalent bacterial genera identified were Streptococcus (probe 4), Lactobacillus (probe 5), Streptococcus (probe 1), and Fusobacterium (probe 4). The most abundant species identified were Lactobacillus salivarius, Streptococcus mutans, Streptococcus sanguinis, and Leptotrichia sp. HOT 417. A comparison of relative species abundance showed a more significant presence of Parascardovia denticolens, S. mutans, and Streptococcus sobrinus in extensive cavitated carious lesions (p < 0.001). The oral biofilm exhibited richness in bacterial species, as the α-Shannon index of 3.02 (SD 0.36) indicated.
CONCLUSION: The oral microbiome in the cariogenic dental biofilm in children from vulnerable communities in Cartagena shows high bacterial diversity, with specific species significantly associated with extensive cavitated lesions.
CLINICAL SIGNIFICANCE: Understanding the microbial diversity and specific bacterial associations in carious lesions provides valuable insights for developing targeted preventive and therapeutic strategies.
HOW TO CITE THIS ARTICLE: Fang L, Herrera-Herrera A, Harris-Ricardo J, et al. Exploring the Oral Microbiome in the Dental Biofilm of Children with Caries from Vulnerable Communities in Cartagena, Colombia: A Cross-sectional Study. Int J Clin Pediatr Dent 2025;18(10):1211-1216.