An Antibiotic-Free Model of Candida albicans Colonization of the Murine Gastrointestinal Tract.

The human gastrointestinal (GI) tract is home to trillions of commensal organisms. Some of these microbes have the capacity to become pathogenic following changes in the microenvironment and/or host physiology. Candida albicans is one such organism, usually inhabiting the GI tract as a harmless commensal in most individuals but with the potential to cause serious infection. Risk factors for C. albicans GI infections include the use of antibiotics, neutropenia, and abdominal surgery. Understanding how commensal organisms can transform into life-threatening pathogens is an important area of research. Mouse models of fungal GI colonization provide an essential platform to study the mechanisms involved in the transition of C. albicans from benign commensal to dangerous pathogen. This chapter presents a novel method of stable, long-term colonization of the murine GI tract with Candida albicans.