Multiple sclerosis (Houndmills, Basingstoke, England) · 2015
Distinct functionality of neutrophils in multiple sclerosis and neuromyelitis optica.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Hertwig Laura, Pache Florence, Romero-Suarez Silvina, Stürner Klarissa H, Borisow Nadja, Behrens Janina, Bellmann-Strobl Judith, Seeger Bibiane, Asselborn Natascha, Ruprecht Klemens, Millward Jason M, Infante-Duarte Carmen, Paul Friedemann
Аннотация
BACKGROUND: In contrast to multiple sclerosis (MS), lesions in neuromyelitis optica (NMO) frequently contain neutrophils. However, the phenotypic profile of neutrophils in these two distinct pathologies remains unknown.
OBJECTIVE: Our aim is to better understand the potential contribution of neutrophils to NMO and MS pathology.
METHODS: We performed the first functional analysis of blood neutrophils in NMO and MS, including evaluation of neutrophil immune response (fMLP receptor, TLR2), chemotaxis and migration (CXCR1, CD62L, CD43), regulation of complement (CD46, CD55, CD59), respiratory burst, phagocytosis and degranulation.
RESULTS: Compared with healthy controls (HC), neutrophils in NMO and MS show an activated phenotype characterized by an increased surface expression of TLR2 and fMLP receptor. However, contrary to MS neutrophils, NMO neutrophils show reduced adhesion and migratory capacity as well as decreased reduced production of reactive oxygen species (respiratory burst) and degranulation.
CONCLUSION: Although NMO and MS neutrophils display an activated phenotype in comparison with HC, NMO neutrophils show a compromised functionality when compared with MS patients. These results suggest a distinct functional profile of neutrophils in MS and NMO.
OBJECTIVE: Our aim is to better understand the potential contribution of neutrophils to NMO and MS pathology.
METHODS: We performed the first functional analysis of blood neutrophils in NMO and MS, including evaluation of neutrophil immune response (fMLP receptor, TLR2), chemotaxis and migration (CXCR1, CD62L, CD43), regulation of complement (CD46, CD55, CD59), respiratory burst, phagocytosis and degranulation.
RESULTS: Compared with healthy controls (HC), neutrophils in NMO and MS show an activated phenotype characterized by an increased surface expression of TLR2 and fMLP receptor. However, contrary to MS neutrophils, NMO neutrophils show reduced adhesion and migratory capacity as well as decreased reduced production of reactive oxygen species (respiratory burst) and degranulation.
CONCLUSION: Although NMO and MS neutrophils display an activated phenotype in comparison with HC, NMO neutrophils show a compromised functionality when compared with MS patients. These results suggest a distinct functional profile of neutrophils in MS and NMO.
Предметные рубрики MeSH
AdultCD55 AntigensCD59 AntigensCase-Control StudiesCell DegranulationChemotaxis, LeukocyteFemaleHumansImmunity, InnateL-SelectinLeukosialinMaleMembrane Cofactor ProteinMiddle AgedMultiple SclerosisNeuromyelitis OpticaNeutrophilsPhagocytosisPhenotypeReceptors, Formyl PeptideReceptors, Interleukin-8ARespiratory BurstToll-Like Receptor 2