Hang tian yi xue yu yi xue gong cheng = Space medicine & medical engineering · 2003
[Effects of NO synthases inhibitor on cardiovascular deconditioning in rats after 7 d tail-suspension].
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Wang De-sheng, Liang Wen-bin, Yuan Ming, Liu Zhao-xia, Zhang Shu-jing, Li Zhi-li
Аннотация
OBJECTIVE: To disclose the changes in pathophysiology of cardiovascular deconditioning (CVD) induced by tail-suspension (TS) and to explore the effects of NOS inhibitor on CVD in TS rats.
METHOD: -30 degrees tail suspended rats were used as the model to simulate the physiological effects of microgravity. Wistar rats were randomly arranged into: control group (CON), 7 d TS group (TS 7 d) and TS 7 d + NOS inhibitor group (TS 7 d + L-NAME). Then, all the rats were subjected to +90 degrees head-up tilt to simulate the physiological effects of orthostatic stress (SO) after 7 d tail-suspension, only that the TS 7d + L-NAME rats were injected with 10(-6) M L-NAME (N-nitro-L-arginine methylester) through hypoglossal vein before tilting after 7 d TS. Carotid pressure and limb II ECG were measured with a PowerLab physiological system during 120 min tilt, and mean arterial pressure (MAP) and heart rate (HR) were analysized.
RESULT: Only one transient episode of hypotension-bradycardia appeared during the tilt in CON rats, but seven episodes occurred in TS 7 d rats, while the TS 7 d + L-NAME rats has only a significant increase in MAP at 10 min. No differences were found between the other parameters in recumbent position and those during the tilt.
CONCLUSION: The CVD in the rats induced by TS is relative to an increase of NO production. 10(-6) M L-NAME had an alleviating effects on the CVD, which might be due to the increase of peripheral resistance of blood vessels and the central sympathetic drive by the combination of L-NAME with NOS. The contribution of the perivascular nerves and pulmonary arteries to this effect remains to be further studied.
METHOD: -30 degrees tail suspended rats were used as the model to simulate the physiological effects of microgravity. Wistar rats were randomly arranged into: control group (CON), 7 d TS group (TS 7 d) and TS 7 d + NOS inhibitor group (TS 7 d + L-NAME). Then, all the rats were subjected to +90 degrees head-up tilt to simulate the physiological effects of orthostatic stress (SO) after 7 d tail-suspension, only that the TS 7d + L-NAME rats were injected with 10(-6) M L-NAME (N-nitro-L-arginine methylester) through hypoglossal vein before tilting after 7 d TS. Carotid pressure and limb II ECG were measured with a PowerLab physiological system during 120 min tilt, and mean arterial pressure (MAP) and heart rate (HR) were analysized.
RESULT: Only one transient episode of hypotension-bradycardia appeared during the tilt in CON rats, but seven episodes occurred in TS 7 d rats, while the TS 7 d + L-NAME rats has only a significant increase in MAP at 10 min. No differences were found between the other parameters in recumbent position and those during the tilt.
CONCLUSION: The CVD in the rats induced by TS is relative to an increase of NO production. 10(-6) M L-NAME had an alleviating effects on the CVD, which might be due to the increase of peripheral resistance of blood vessels and the central sympathetic drive by the combination of L-NAME with NOS. The contribution of the perivascular nerves and pulmonary arteries to this effect remains to be further studied.