Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie · 2026
Vascular remodeling and microvessel density in inflammatory radicular cysts: a CD34 immunohistochemical analysis.
СтатьяНазвание и аннотация: Перевод в очереди
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Iacov Alexandra Cătălina, Camen Adrian, Munteanu Maria Cristina, Diaconu Oana Andreea, Popescu Alexandru Dan, Boldeanu Gabriela, Liliac Ilona Mihaela, Andrei Elena Cristina, Mogoantă Carmen Aurelia, Dascălu Ionela Teodora, Pătru Larisa, Ţuculină Mihaela Jana
Аннотация
BACKGROUND: Inflammatory radicular cysts are lesions of endodontic origin in which chronic inflammation and tissue remodeling may support cyst expansion.
AIM: The present study evaluated cluster of differentiation 34 (CD34) immunoexpression as a proxy of vascular remodeling and explored a digital, artificial intelligence (AI)-assisted quantification workflow.
MATERIALS AND METHODS: Thirty-nine inflammatory radicular cysts were processed as formalin-fixed paraffin-embedded (FFPE) specimens and immunostained with anti-CD34 antibody. Digital quantification [Deep Learning Inferred ImmunoFluorescence (DeepLIIF) and ImageJ] was performed in selected stromal areas. Lesions were stratified by cyst size (0.5-1.0 cm, 1.0-2.0 cm, >2.0 cm).
RESULTS: Twenty-two of 39 cases were CD34-positive. In these cases, the mean proportion of CD34-positive nuclei increased with lesion size (6.67% in 0.5-1.0 cm, 15.98% in 1.0-2.0 cm, and 31.13% in >2.0 cm). Differences among size groups were significant (Kruskal-Wallis H=18.50, p=0.000096).
CONCLUSIONS: Larger inflammatory radicular cysts displayed higher CD34-associated vascular signal, supporting an association between cyst size and vascular remodeling.
AIM: The present study evaluated cluster of differentiation 34 (CD34) immunoexpression as a proxy of vascular remodeling and explored a digital, artificial intelligence (AI)-assisted quantification workflow.
MATERIALS AND METHODS: Thirty-nine inflammatory radicular cysts were processed as formalin-fixed paraffin-embedded (FFPE) specimens and immunostained with anti-CD34 antibody. Digital quantification [Deep Learning Inferred ImmunoFluorescence (DeepLIIF) and ImageJ] was performed in selected stromal areas. Lesions were stratified by cyst size (0.5-1.0 cm, 1.0-2.0 cm, >2.0 cm).
RESULTS: Twenty-two of 39 cases were CD34-positive. In these cases, the mean proportion of CD34-positive nuclei increased with lesion size (6.67% in 0.5-1.0 cm, 15.98% in 1.0-2.0 cm, and 31.13% in >2.0 cm). Differences among size groups were significant (Kruskal-Wallis H=18.50, p=0.000096).
CONCLUSIONS: Larger inflammatory radicular cysts displayed higher CD34-associated vascular signal, supporting an association between cyst size and vascular remodeling.