Circulating antibodies in rabbits against a Kveim Material.

For 1 year five rabbits were sensitized with a solubilized Kveim material. After 50 days, precipitating antibodies were demonstrated by passive immunodiffusion. Crossed immunoelectrophoresis using Kveim material against concentrated rabbit serum showed ill-defined precipitates, while two well-defined precipitates were seen when rocket immunoelectrophoresis was used. One of the precipitates probably represented an antibody against human serum protein in the K veim material, while the other may have been a specific antibody against the K veim material. No precipitates were seen when rocket immunoelectrophoresis was applied to sera from two patients with active sarcoidosis.