Use of a PCR assay for diagnosing African trypanosomiasis of the CNS: a case report.

The diagnosis of African trypanosomiasis is parasitologic and often can be difficult, especially in patients infected with Trypanosoma brucei gambiense, the cause of West African sleeping sickness. In the United States imported cases of sleeping sickness are rare, and most occur in tourists returning from East African game parks rather than among immigrants. I report here the use of a T. brucei specific PCR assay in a West African immigrant who presented with neurological symptoms more than 12 years after he had last been in Africa. The patient's historical and physical findings, as well as abnormal cerebrospinal fluid (CSF) parameters, suggested a diagnosis of sleeping sickness. The diagnosis was confirmed when the PCR assay demonstrated the presence of parasite DNA in CSF and blood. Several months after curative therapy the CSF continued to be positive by PCR. These findings suggest that the PCR assay may be useful for sensitive and specific diagnosis of sleeping sickness, but that it may not be helpful for assessing the effect of drug treatment.