Subtractive proteomics to identify targets for vaccine development against vancomycin-resistant Enterococcus faecalis.

Aim: Due to the increased level of vancomycin resistance in Enterococci species, an aggressive treatment involving targeted antibiotics is required to manage this frequently occurring infection. Materials & methods: Here, subtractive proteomics and reverse vaccinology approaches were employed to identify potential target and for the prediction of B cell and T cell epitopes against vancomycin-resistant Enterococcus faecalis (VRE V583).

Results

The results exhibited the presence of 73 out of 805 non-homologous protein sequences in the proteome which can be employed as unique targets to develop the novel drugs and vaccine to counter the deadly infections caused by this microbe.

Conclusion

The identified novel target in VRE V583 will equip our knowledge to design effective vaccine against probable protease EEP proteins.