European journal of nuclear medicine and molecular imaging · 2020
Molecular detection of Gram-positive bacteria in the human lung through an optical fiber-based endoscope.
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Mills Bethany, Megia-Fernandez Alicia, Norberg Dominic, Duncan Sheelagh, Marshall Adam, Akram Ahsan R, Quinn Thomas, Young Irene, Bruce Annya M, Scholefield Emma, Williams Gareth O S, Krstajić Nikola, Choudhary Tushar R, Parker Helen E, Tanner Michael G, Harrington Kerrianne, Wood Harry A C, Birks Timothy A, Knight Jonathan C, Haslett Christopher, Dhaliwal Kevin, Bradley Mark, Ucuncu Muhammed, Stone James M
Аннотация
PURPOSE: The relentless rise in antimicrobial resistance is a major societal challenge and requires, as part of its solution, a better understanding of bacterial colonization and infection. To facilitate this, we developed a highly efficient no-wash red optical molecular imaging agent that enables the rapid, selective, and specific visualization of Gram-positive bacteria through a bespoke optical fiber-based delivery/imaging endoscopic device.
METHODS: We rationally designed a no-wash, red, Gram-positive-specific molecular imaging agent (Merocy-Van) based on vancomycin and an environmental merocyanine dye. We demonstrated the specificity and utility of the imaging agent in escalating in vitro and ex vivo whole human lung models (n = 3), utilizing a bespoke fiber-based delivery and imaging device, coupled to a wide-field, two-color endomicroscopy system.
RESULTS: The imaging agent (Merocy-Van) was specific to Gram-positive bacteria and enabled no-wash imaging of S. aureus within the alveolar space of whole ex vivo human lungs within 60 s of delivery into the field-of-view, using the novel imaging/delivery endomicroscopy device.
CONCLUSION: This platform enables the rapid and specific detection of Gram-positive bacteria in the human lung.
METHODS: We rationally designed a no-wash, red, Gram-positive-specific molecular imaging agent (Merocy-Van) based on vancomycin and an environmental merocyanine dye. We demonstrated the specificity and utility of the imaging agent in escalating in vitro and ex vivo whole human lung models (n = 3), utilizing a bespoke fiber-based delivery and imaging device, coupled to a wide-field, two-color endomicroscopy system.
RESULTS: The imaging agent (Merocy-Van) was specific to Gram-positive bacteria and enabled no-wash imaging of S. aureus within the alveolar space of whole ex vivo human lungs within 60 s of delivery into the field-of-view, using the novel imaging/delivery endomicroscopy device.
CONCLUSION: This platform enables the rapid and specific detection of Gram-positive bacteria in the human lung.