The Journal of clinical pediatric dentistry · 2011
Dental pulp stem cells from primary teeth quality analysis: laboratory procedures.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Saha R, Tandon S, Rajendran R, Nayak R
Аннотация
OBJECTIVES: To present details of isolation, processing and differentiation of stem cells from inflamed dental pulp of primary teeth.
MATERIALS AND METHODS: Tissue sample was collected from teeth indicated for a single visit pulp therapy. Samples were transported and processed in the laboratory which included culturing of cells, isolation and in vitro differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow.
RESULTS: There was no statistically significant difference found in the expression of various surface markers between dental pulp and bone marrow. The stem cells from dental pulp were differentiated into multiple lineages.
CONCLUSION: Isolation of cells from oral tissues is technique sensitive.
MATERIALS AND METHODS: Tissue sample was collected from teeth indicated for a single visit pulp therapy. Samples were transported and processed in the laboratory which included culturing of cells, isolation and in vitro differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow.
RESULTS: There was no statistically significant difference found in the expression of various surface markers between dental pulp and bone marrow. The stem cells from dental pulp were differentiated into multiple lineages.
CONCLUSION: Isolation of cells from oral tissues is technique sensitive.