Indian journal of medical microbiology · 2025
Transmission dynamics of multidrug resistant Klebsiella pneumoniae from an Indian hospital.
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Puraswani Mamta, Ashita Ashita, Kumari Ambashta Neha, Aggrawal Richa, Soni Kapil Dev, Kumar Subodh, Sagar Sushma, Gupta Amit, Farooque Kamran, Mathur Purva
Аннотация
OBJECT: The dissemination of Klebsiella pneumoniae is becoming a major concern, as this organism is responsible for a significant proportion of hospital acquired infections. Due to complexity of this organism adequate knowledge on the epidemiology and infection control practices associated with the dissemination is highly required. Therefore, this study designed to include consecutive samples from environment and patients to assess the similarity pattern among isolates from different sources.
METHODOLOGY: We included patients with hospital acquired infections with K. pneumonaie and environment isolates. To obtain the complete sequence forty-eight isolates were sequenced on Illumina MiSeq 250 2 × 250 bp paired end (Illumina, USA) and Nanopore (Oxford). These includes 30 BSI cases, 13 environment and 5 from water. Assembly of good quality reads were prepared using Unicycler. AMR gene detection was done using Resfinder of Abricate and sequence similarity was observed by SNP based phylogenetic analysis.
RESULTS: The most common sequence type of organism among all was ST 231 and ST 395. ST 29 was common between water and clinical isolates. Average (range) number of AMR genes present in clinical isolates were 16 (3-24). The antimicrobial genes belong to 41 classes and fosA was highly prevalent. The frequency of blaNDM was present 55.4 % (27/48) and blaOXA in 61.6 % (30/48). The strain ST 395 and ST 16 carried highest no. of replicons n = 10 and n = 9. The predominant plasmid replicon Col440I (N = 58) followed by IncFII_1_pKP91 (N = 34) and ColRNAI (N = 29). The phylogenetic analysis showed high similarity between clinical and environmental samples.
CONCLUSION: This study concludes that environment play essential role in disseminating the infectious strains of organism resulting in increased rates of hospital acquired infections. Therefore, there is an imperative necessity for implementing infection control practices to prevent the spread of infectious diseases.
METHODOLOGY: We included patients with hospital acquired infections with K. pneumonaie and environment isolates. To obtain the complete sequence forty-eight isolates were sequenced on Illumina MiSeq 250 2 × 250 bp paired end (Illumina, USA) and Nanopore (Oxford). These includes 30 BSI cases, 13 environment and 5 from water. Assembly of good quality reads were prepared using Unicycler. AMR gene detection was done using Resfinder of Abricate and sequence similarity was observed by SNP based phylogenetic analysis.
RESULTS: The most common sequence type of organism among all was ST 231 and ST 395. ST 29 was common between water and clinical isolates. Average (range) number of AMR genes present in clinical isolates were 16 (3-24). The antimicrobial genes belong to 41 classes and fosA was highly prevalent. The frequency of blaNDM was present 55.4 % (27/48) and blaOXA in 61.6 % (30/48). The strain ST 395 and ST 16 carried highest no. of replicons n = 10 and n = 9. The predominant plasmid replicon Col440I (N = 58) followed by IncFII_1_pKP91 (N = 34) and ColRNAI (N = 29). The phylogenetic analysis showed high similarity between clinical and environmental samples.
CONCLUSION: This study concludes that environment play essential role in disseminating the infectious strains of organism resulting in increased rates of hospital acquired infections. Therefore, there is an imperative necessity for implementing infection control practices to prevent the spread of infectious diseases.