The Journal of antimicrobial chemotherapy · 2025
Pandemic human-associated extended-spectrum β-lactamase-producing Escherichia coli lineages of ST38, ST131 and ST141 identified in Viennese dogs.
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Saria Pia, Doulidis Pavlos G, Desvars-Larrive Amélie, Tóth Adrienn Gréta, Burgener Iwan A, Rodríguez-Rojas Alexandro, Makarova Olga
Аннотация
OBJECTIVES: To assess the prevalence of ESBL Enterobacteriaceae among dogs attending a veterinary clinic in Vienna, characterize the isolates in terms of antimicrobial resistance, virulence and phylogenetic relationships.
METHODS: Faecal samples of 88 dogs were streaked on selective plates, species were identified by MALDI-ToF MS, tested for resistance by a combination disk test and VITEK 2®, whole genome-sequenced, bioinformatically genotyped, phylogenetically analysed and screened for resistance and virulence genes.
RESULTS: ESBL Escherichia coli carriage rate was 14.8% (95% CI: [8.1-23.9]). No carbapenem resistance was found, but 53.8% of the isolates were classified genotypically as multi-drug resistant. Phylogenetic analyses revealed that half of the isolates belonged to animal and environment-associated phylogroups, while another half was human-associated, and included high-risk international clones of ST38, ST131 and ST141, which clustered primarily with human isolates. All isolates harboured various virulence-associated genes, including four isolates that encoded exotoxins, of which two were from the pandemic ST131 and emerging ST141 lineages.
CONCLUSIONS: Dogs in Vienna carry ESBL E. coli with high rates of multi-drug resistance and virulence, and a highly diverse population structure that includes pandemic human-associated lineages.
METHODS: Faecal samples of 88 dogs were streaked on selective plates, species were identified by MALDI-ToF MS, tested for resistance by a combination disk test and VITEK 2®, whole genome-sequenced, bioinformatically genotyped, phylogenetically analysed and screened for resistance and virulence genes.
RESULTS: ESBL Escherichia coli carriage rate was 14.8% (95% CI: [8.1-23.9]). No carbapenem resistance was found, but 53.8% of the isolates were classified genotypically as multi-drug resistant. Phylogenetic analyses revealed that half of the isolates belonged to animal and environment-associated phylogroups, while another half was human-associated, and included high-risk international clones of ST38, ST131 and ST141, which clustered primarily with human isolates. All isolates harboured various virulence-associated genes, including four isolates that encoded exotoxins, of which two were from the pandemic ST131 and emerging ST141 lineages.
CONCLUSIONS: Dogs in Vienna carry ESBL E. coli with high rates of multi-drug resistance and virulence, and a highly diverse population structure that includes pandemic human-associated lineages.