Bioorganic & medicinal chemistry letters · 2005
Synthesis and bioactivities of two multiple antigen peptides as potential vaccine against schistosoma.
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Huang He-Qing, Li Shu-Chun, Qin Zhi-Hui, Cao Sheng-Li, Yao Yun, Liu Yu-Shi, Li Huai-Yu, Cai Meng-Shen, Li Zhong-Jun, Shi You-En
Аннотация
Based on the two antigenic peptides, 26-43 (P26) and 116-131 (P116), derived from 28 kDa glutathione S-transferase of Schistosoma mansoni (Sm28GST), two multiple antigenic peptides (MAPs), (P26)4-MAP and (P116)4-MAP with the same oligomeric lysine core, were synthesized by stepwise solid-phase peptide synthesis method. The antigenicities and protective effects of these two MAPs were examined on experimental animals. As shown in the dot-ELISA result, the synthetic MAPs could be recognized and bound by immunoglobins in both patient's and infected-rabbit's sera. After Kunming mice were immunized with (P26)4-MAP, the worm burden reduction rate and the liver egg reduction rate were 59.9% and 61.1%. In (P26)4-MAP or (P116)4-MAP immunized BALB/c mice, the worm burden reduction rates were 37.5% and 62.5%, respectively, and the liver egg reduction rates were 35.1% and 54.0%, respectively.