[Effect of dietary protein and vitamin B group deficiencies on the calcium-binding protein content in the mucosa of the small intestine].

The content of calcium-binding protein (CBP) and calcium-binding activity of small intestinal mucoproteins were examined in growing male rats kept on the diet deficient in proteins and group B vitamins (thiamine, riboflavin and pyridoxine). CBP was isolated by means of electrophoresis in 7.5% polyacrylamide gel. Calcium-binding activity of mucoproteins was explored with the aid of dialysis followed by complexonometric titration. The deficiency of food proteins was shown to lead to substantial changes in the content of vitamin D-dependent CBP and calcium-binding activity of mucoproteins. The deficiency of group B vitamins did not produce any appreciable influence on the content of CBP or calciumbinding activity of total mucoproteins.