Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2026
[Silybin inhibits fibrosis after glaucoma filtration surgery in rabbits by promoting fibroblast autophagy].
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Zhang Yuyang, Shen Ying, Tong Xinyu, Duan Yukui, Luo Yunna, Guo Wenqi
Аннотация
OBJECTIVES: To investigate the effect of silybin in inhibiting fibrosis after glaucoma filtration surgery and the underlying mechanism.
METHODS: Twenty-five healthy rabbits undergoing unilateral trabeculectomy on the left eye were randomly assigned into 5 groups for treatment with subconjunctival injections of sterilized water (control) or 50, 100, 200, and 250 μmol/L silybin for 7 consecutive days. Postoperative intraocular pressure (IOP) and filtration bleb morphology (Krofeld classification) were monitored. On day 28, tissues samples were harvested from the operated eyes for HE staining, Masson's trichrome staining, and immunofluorescence detection of fibronectin and collagen I. In cultured rabbit Tenon's capsule fibroblasts with TGF‑β1-induced fibrosis, the effects of silybin on autophagy and apoptosis were analyzed using Western blotting (LC3II/LC3I ratio and p62 expression) and flow cytometry.
RESULTS: From postoperative day 7 to day 21, the rabbits with silybin treatment showed significantly lower IOP than those in the control group, and in the 200 and 250 μmol/L silybin groups, IOP reduction was maintained up to postoperative day 28. From postoperative day 14 to day 21, silybin dose-dependently increased the formation rate of functional filtering blebs, the 200 and 250 μmol/L silybin groups maintained more than 40% functional filtering blebs from postoperative day 14 to day 28. HE staining revealed obviously lessened inflammatory cell infiltration in silybin treatment groups compared with that in the control group. Masson's trichrome staining showed progressively reduced fibroblast numbers and collagen deposition in the silybin groups. Immunofluorescence staining confirmed that silybin dose-dependently reduced fibronectin and collagen I-positive cells. In cultured rabbit Tenon's capsule fibroblasts, silybin treatment effectively reversed TGF-β1-induced fibroblast fibrotic phenotype, increased the LC3II/LC3I ratio, decreased p62 expression, and promoted apoptosis of the fibroblasts.
CONCLUSIONS: Silybin significantly inhibits postoperative fibrosis in rabbit models of glaucoma likely by activating cellular autophagy and inducing apoptosis to reduce fibroblast activation.
METHODS: Twenty-five healthy rabbits undergoing unilateral trabeculectomy on the left eye were randomly assigned into 5 groups for treatment with subconjunctival injections of sterilized water (control) or 50, 100, 200, and 250 μmol/L silybin for 7 consecutive days. Postoperative intraocular pressure (IOP) and filtration bleb morphology (Krofeld classification) were monitored. On day 28, tissues samples were harvested from the operated eyes for HE staining, Masson's trichrome staining, and immunofluorescence detection of fibronectin and collagen I. In cultured rabbit Tenon's capsule fibroblasts with TGF‑β1-induced fibrosis, the effects of silybin on autophagy and apoptosis were analyzed using Western blotting (LC3II/LC3I ratio and p62 expression) and flow cytometry.
RESULTS: From postoperative day 7 to day 21, the rabbits with silybin treatment showed significantly lower IOP than those in the control group, and in the 200 and 250 μmol/L silybin groups, IOP reduction was maintained up to postoperative day 28. From postoperative day 14 to day 21, silybin dose-dependently increased the formation rate of functional filtering blebs, the 200 and 250 μmol/L silybin groups maintained more than 40% functional filtering blebs from postoperative day 14 to day 28. HE staining revealed obviously lessened inflammatory cell infiltration in silybin treatment groups compared with that in the control group. Masson's trichrome staining showed progressively reduced fibroblast numbers and collagen deposition in the silybin groups. Immunofluorescence staining confirmed that silybin dose-dependently reduced fibronectin and collagen I-positive cells. In cultured rabbit Tenon's capsule fibroblasts, silybin treatment effectively reversed TGF-β1-induced fibroblast fibrotic phenotype, increased the LC3II/LC3I ratio, decreased p62 expression, and promoted apoptosis of the fibroblasts.
CONCLUSIONS: Silybin significantly inhibits postoperative fibrosis in rabbit models of glaucoma likely by activating cellular autophagy and inducing apoptosis to reduce fibroblast activation.