[Intravital and electron microscopic analysis of the arterioles by immersion and perfusion technics of fixation of the rat mesentery].

The technique of the intravital microscopic control, videotaping, cinemoregistration and per single-frame analysis made it possible to demonstrate for the first time that a double fixation in glutaraldehyde and osmium has no effect on the arteriolar lumen. Comparing the data on the intravital and a detailed electron microscopic study, it was possible to reveal certain peculiarities and advantages of the fixative perfusion and immersion techniques applied. The investigation establishes a basis for extended morphological studies of small blood vessels and morpho-physiological comparisons.