The Plant cell · 2022
Dwarf and High Tillering1 represses rice tillering through mediating the splicing of D14 pre-mRNA.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Liu Tianzhen, Zhang Xin, Zhang Huan, Cheng Zhijun, Liu Jun, Zhou Chunlei, Luo Sheng, Luo Weifeng, Li Shuai, Xing Xinxin, Chang Yanqi, Shi Cuilan, Ren Yulong, Zhu Shanshan, Lei Cailin, Guo Xiuping, Wang Jie, Zhao Zhichao, Wang Haiyang, Zhai Huqu, Lin Qibing, Wan Jianmin
Аннотация
Strigolactones (SLs) constitute a class of plant hormones that regulate many aspects of plant development, including repressing tillering in rice (Oryza sativa). However, how SL pathways are regulated is still poorly understood. Here, we describe a rice mutant dwarf and high tillering1 (dht1), which exhibits pleiotropic phenotypes (such as dwarfism and increased tiller numbers) similar to those of mutants defective in SL signaling. We show that DHT1 encodes a monocotyledon-specific hnRNP-like protein that acts as a previously unrecognized intron splicing factor for many precursor mRNAs (pre-mRNAs), including for the SL receptor gene D14. We find that the dht1 (DHT1I232F) mutant protein is impaired in its stability and RNA binding activity, causing defective splicing of D14 pre-mRNA and reduced D14 expression, and consequently leading to the SL signaling-defective phenotypes. Overall, our findings deepen our understanding of the functional diversification of hnRNP-like proteins and establish a connection between posttranscriptional splicing and SL signaling in the regulation of plant development.