Phytomedicine : international journal of phytotherapy and phytopharmacology · 2025
A xanthone O-glucoside isolated from Iris setosa Pall. ex Link exhibits promising anti-amoebic activity against the brain-eating amoeba Naegleria fowleri.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Lê Hương Giang, Hwang Buyng Su, Choi Ji-Su, Jeong Yong Tae, Võ Tuấn Cường, Cho Minkyoung, Hong Yeonchul, Kim Jeong Ho, Oh Young Taek, Na Byoung-Kuk
Аннотация
BACKGROUND: Primary amoebic meningoencephalitis (PAM), caused by Naegleria fowleri, poses a significant challenge in humans owing to its high mortality rate, exceeding 97 %. Current therapeutics have been unable to deliver satisfactory treatment outcomes, due to suboptimal efficacy and toxicity, highlighting the critical need for efficacious drug development. In this study, we identified a natural compound, 3,5-Dihydroxy-8‑methoxy-1-O-β-d-glucopyranosyl xanthone (DX), which exhibited promising anti-N. fowleri activity, and investigated its underlying anti-N. fowleri mechanisms.
METHODS: The anti-amoebic activity of DX against N. fowleri trophozoites and its cytotoxicity against C6 glial cells were evaluated using a cell viability assay. The anti-N. fowleri mechanism of DX involved analyses of apoptosis-necrosis, TUNEL, caspase-3, ROS production, mitochondrial dysfunction, ATP production, qRT-PCR, and actin integrity. The efficacy of DX in protecting C6 glial cells from the amoebae was assessed using a co-culture method.
RESULTS: DX, isolated from Iris setosa Pall. ex Link, demonstrated significant anti-N. fowleri activity with an IC50 of 55.37 ± 2.58 µM, exhibiting a high selectivity index (> 9.03) and negligible cytotoxicity to C6 glial cells. DX induced apoptosis-like programmed cell death in the amoebae, characterized by DNA fragmentation, increased caspase-3 activity, and mitochondrial dysfunction. DX also disrupted actin cytoskeletal integrity in the amoebae. DX significantly protected C6 glial cells against phagocytosis of N. fowleri trophozoites.
CONCLUSION: The selective anti-amoebic activity of DX for N. fowleri underscores its potential as a promising candidate drug or a supplemental compound in the development or optimization of therapeutic drugs for PAM.
METHODS: The anti-amoebic activity of DX against N. fowleri trophozoites and its cytotoxicity against C6 glial cells were evaluated using a cell viability assay. The anti-N. fowleri mechanism of DX involved analyses of apoptosis-necrosis, TUNEL, caspase-3, ROS production, mitochondrial dysfunction, ATP production, qRT-PCR, and actin integrity. The efficacy of DX in protecting C6 glial cells from the amoebae was assessed using a co-culture method.
RESULTS: DX, isolated from Iris setosa Pall. ex Link, demonstrated significant anti-N. fowleri activity with an IC50 of 55.37 ± 2.58 µM, exhibiting a high selectivity index (> 9.03) and negligible cytotoxicity to C6 glial cells. DX induced apoptosis-like programmed cell death in the amoebae, characterized by DNA fragmentation, increased caspase-3 activity, and mitochondrial dysfunction. DX also disrupted actin cytoskeletal integrity in the amoebae. DX significantly protected C6 glial cells against phagocytosis of N. fowleri trophozoites.
CONCLUSION: The selective anti-amoebic activity of DX for N. fowleri underscores its potential as a promising candidate drug or a supplemental compound in the development or optimization of therapeutic drugs for PAM.