PdPtB Electrochemiluminescence Nanoenhancer and SiC@Au-PEDOT Nanowires-Based Detection of β-Amyloid Oligomers in Alzheimer's Disease.

β-Amyloid oligomers (AβOs) are promising biomarkers for the diagnosis of Alzheimer's disease (AD). The present research introduces a novel electrochemiluminescence (ECL) immunosensor based on PdPtB nanoenhancer and SiC@Au-PEDOT nanowires (NWs) for the specific and ultrasensitive detection of AβOs. The PdPtB nanoenhancer exhibited excellent oxidase-like catalytic activity with in situ generation of reactive oxygen species (ROS) to enhance luminol ECL in neutral media. In addition, SiC@Au-PEDOT NWs were utilized as a biocompatible and conductive substrate for the modification of the glassy carbon electrode (GCE). With this design, the ECL immunosensor showed outstanding AβOs analytical performance without exogenous coreactant. The ECL immunosensor demonstrated a favorable linear range of 20 pM to 20 nM and a detection limit of 10 pM under optimized conditions with potential straightforward clinical application. In general, the developed ECL immunosensor provides a promising strategy for the early diagnosis of AD.