[Evaluation of beta-lactamase detection reagent with used HMRZ-86 for the detection of extended-spectrum beta-lactamase and metallo-beta-lactamase].

Currently, evidence related to infectious diseases and the nosocomial infection in hospitals with Gram-negative rods with extended spectrum beta-lactamase (ESBL) or metallo-beta-lactamase (MBL) is accumulating. Thereby, it will be very important to know quickly the resistant mechanisms for antibiotics in the treatment strategy of patients with infectious diseases in a clinical setting. Then, beta-lactamase detection reagent Cica beta-Test (Kanto Chemical, Tokyo) which used HMRZ-86 was evaluated. Bacteria used in the present study were 42 stocked strains of beta-lactamase produced strain isolated from clinical samples in our hospital. ESBL were coincided in 13 strains among 17 (76.5%). MBL were coincided in 8 strains among 11 (72.7%). KOXY belongs to class A beta-lactamase was missjudged as ESBL. Therefore, although there are a little of limitations. Cica beta-Test provides us the rapid information of the esistance mechanism in 15-min with relatively, which is surely valuable to treat patients with bacterial infection and to prevent the nosocomial infection.