Network Pharmacology-Based Characterization of Mecasin (KCHO-1) as a Multi-Target Modulator of Neuroinflammatory Pathways in Alzheimer's Disease.

Background/

Objectives

Mecasin (KCHO-1) is a standardized multi-herb formulation containing diverse bioactive compounds predicted to engage multiple molecular targets. This study applied an integrative network pharmacology approach to explore how Mecasin may interact with Alzheimer's disease (AD)-related molecular networks.

Methods

Bioactive constituents from 9 herbs were screened through OASIS and PubChem, and their predicted targets were cross-referenced with 8886 AD-associated genes from GeneCards. Overlapping genes were analyzed using protein-protein interaction mapping, Gene Ontology, and KEGG to identify potential Mecasin-AD core nodes and pathways. Co-expression, co-regulation, and molecular docking analyses were performed to further characterize mechanistic relevance.

Results

Network integration identified 6 core genes-AKT1, STAT3, IL6, TNF, EGFR, and IL1B-positioned within signaling pathways related to neuronal survival, inflammatory regulation, and cellular stress responses, including FoxO, JAK-STAT, MAPK, and TNF pathways. Molecular docking suggested that several Mecasin compounds may interact with targets such as AKT1 and TNF.

Conclusions

These in silico findings indicate that Mecasin, a multi-component formulation containing numerous phytochemicals that generate broad compound-target associations, may interface with interconnected neuroimmune pathways relevant to AD. While exploratory, the results highlight potential multi-target mechanisms that merit further investigation and provide a systems-level framework to inform future experimental validation.