[3-dimensional reconstruction in dermatology. Prospects for new possibilities in routine histological diagnosis].

BACKGROUND AND

OBJECTIVE

The combination of digital image-analysis and computer-reconstruction of the three-dimensional architecture of the human skin can make this method usable on an acceptable time scale.

PATIENTS/

METHODS

We used biopsies of different diseases (malignant melanoma, lichen planus, psoriasis) and of normal skin. Immunohistologically stained sections were digitized and adjusted with respect to features of interest using the interlaced mode of an commercial image analysis system (Lucia G, Nikon GmbH, Düsseldorf). The sections have been segmented and the resulting three dimensional data sets were visualized on a UNIX based work station.

RESULTS

Three dimensional reconstruction provides a different view into the architecture of normal and diseased skin: for example, the course of the duct of sweat glands or the perforation of a malignant melanoma through the underlying infiltrate.

CONCLUSIONS

A new rapid reconstruction method is presented producing multidimensional histological images of the skin within a few hours. This automation is of interest in basic research and should find its way into routine histological diagnosis.