Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2003
[Preparation and identification of polyclonal antibody to B cell epitope domain of TRP-1].
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Li Ting-Hui, Gao Tian-Wen, Li Chun-Ying, Hou Xiao-Bin, Liu Yu-Feng
Аннотация
AIM: To prepare the polyclonal antibody to B cell epitope domain of TRP-1 and apply it to study the immunotherapy of vitiligo and melanoma.
METHODS: The fusion protein PRSETA/TRP-1 was expressed in E.coli, the polyclonal antibody was prepared by immunizing a rabbit with the protein. The qulity of the antibody was identified by ELISA and Western-blot.
RESULTS: (1)The pRSEA/TRP-1 fusion protein was correctly expressed;(2)the polyclonal antibodies to the B cell eoitope domain of TRP-1 were obtained; (3)the rabbit antiserum containing of polyclonal antibody showed high titer and possessed good binding activity to 6His-TRP1 expressed by Pichia pastoris.
CONCLUSION: The polyclonal antibody could be applied to the research on B cell epitope domain of TRP-1.
METHODS: The fusion protein PRSETA/TRP-1 was expressed in E.coli, the polyclonal antibody was prepared by immunizing a rabbit with the protein. The qulity of the antibody was identified by ELISA and Western-blot.
RESULTS: (1)The pRSEA/TRP-1 fusion protein was correctly expressed;(2)the polyclonal antibodies to the B cell eoitope domain of TRP-1 were obtained; (3)the rabbit antiserum containing of polyclonal antibody showed high titer and possessed good binding activity to 6His-TRP1 expressed by Pichia pastoris.
CONCLUSION: The polyclonal antibody could be applied to the research on B cell epitope domain of TRP-1.