Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control · 2013
[Changes of pyrethroid resistance and P450 monooxygenase activity with age in Anopheles sinensis in Huainan City, Anhui Province, China].
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Xu Tie-Long, Zhong Dai-Bin, Fang Qiang, Tang Lin-Hua, Zhou Shui-Sen, Zheng Bin
Аннотация
OBJECTIVE: To study the effect of mosquito age on the pyrethroid resistance and P450 monooxygenase activity in Anopheles sinensis in Huainan City, Anhui Province, China, as well as the molecular mechanism of pyrethroid resistance.
METHODS: Anopheles mosquito larvae were collected in fields, and were emerged to adults. Then the pyrethroid resistance and P450 monooxygenase activity of female mosquitoes were detected 1, 3, 6, 9, 12, 15 d and 20 d after emergence. Meanwhile, the resistance level and P450 monooxygenase activity of the sensitive mosquitoes in lab were detected as the control group.
RESULTS: curves of pyrethroid resistance and P450 monooxygenase activity changes with the mosquito age presented unsymmetrically reversed "U" type. At 1 d after emergence, the resistance level and P450 monooxygenase activity were lower, and then they increased quickly. When 3-9 d after emergence, the resistance level and P450 monooxygenase activity rose to the highest level, and entered into plateau, then declined gradually. Compared to the sensitive mosquitoes in lab, the P450 monooxygenase activity of those in the field was higher at 3-12 d after emergence, but lower at 1 d after emergence, and at 15 and 20 d after emergence, they were almost equal to each other.
CONCLUSION: The mosquito age is a confounding factor in the detection of resistance level and enzyme activity. The promotion of activity of P450 monooxygenase may be one of the reasons of the resistance. Using female adults with different ages in the field as study subjects may underestimate the real resistance level of mosquitoes, and mislead the use of insecticides.
METHODS: Anopheles mosquito larvae were collected in fields, and were emerged to adults. Then the pyrethroid resistance and P450 monooxygenase activity of female mosquitoes were detected 1, 3, 6, 9, 12, 15 d and 20 d after emergence. Meanwhile, the resistance level and P450 monooxygenase activity of the sensitive mosquitoes in lab were detected as the control group.
RESULTS: curves of pyrethroid resistance and P450 monooxygenase activity changes with the mosquito age presented unsymmetrically reversed "U" type. At 1 d after emergence, the resistance level and P450 monooxygenase activity were lower, and then they increased quickly. When 3-9 d after emergence, the resistance level and P450 monooxygenase activity rose to the highest level, and entered into plateau, then declined gradually. Compared to the sensitive mosquitoes in lab, the P450 monooxygenase activity of those in the field was higher at 3-12 d after emergence, but lower at 1 d after emergence, and at 15 and 20 d after emergence, they were almost equal to each other.
CONCLUSION: The mosquito age is a confounding factor in the detection of resistance level and enzyme activity. The promotion of activity of P450 monooxygenase may be one of the reasons of the resistance. Using female adults with different ages in the field as study subjects may underestimate the real resistance level of mosquitoes, and mislead the use of insecticides.