Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control · 2012
[Field evaluation of IgG antibody detection kits of Schistosoma japonicum and quality control analysis].
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Chu Yan-Hong, Ai Lin, Yao Jun-Min, Guo Jian, Cai Yu-Chun, Chen Jia-Xu
Аннотация
OBJECTIVE: To evaluate the application effect of an ELISA kit for detection of IgG antibody to Schistosomajaponicum in endemic areas.
METHODS: In two schistosomiasis endemic villages of Eryuan County, Yunnan Province, there were 505 permanent residents enrolled in the study. Fecal examinations were carried out by using the nylon screen bag-egg concentration method. In paralleled testing, the ELISA kit was used for the villagers to detect the IgG antibody to S. japonicum. The assay was performed daily for 20 consecutive days, and the data were analyzed by comparison with the parasitological examination and by using the L - J quality control chart method.
RESULTS: In 505 examinees, 290 were positive by ELISA, with a positive rate of 57.43%. The L-J chart analysis showed that the deviation of A450 value between the quality control sera A and B was within the controllable range. The fecal examination found 20 positives with a positive rate 3.96%. Compared with the fecal examination, the positive consistency rate of the ELISA method reached to 90.00%.
CONCLUSION: The ELISA kit for detection of IgG antibody to S. japonicum is stable, reliable and suitable for the screening of schistosomiasis in the field.
METHODS: In two schistosomiasis endemic villages of Eryuan County, Yunnan Province, there were 505 permanent residents enrolled in the study. Fecal examinations were carried out by using the nylon screen bag-egg concentration method. In paralleled testing, the ELISA kit was used for the villagers to detect the IgG antibody to S. japonicum. The assay was performed daily for 20 consecutive days, and the data were analyzed by comparison with the parasitological examination and by using the L - J quality control chart method.
RESULTS: In 505 examinees, 290 were positive by ELISA, with a positive rate of 57.43%. The L-J chart analysis showed that the deviation of A450 value between the quality control sera A and B was within the controllable range. The fecal examination found 20 positives with a positive rate 3.96%. Compared with the fecal examination, the positive consistency rate of the ELISA method reached to 90.00%.
CONCLUSION: The ELISA kit for detection of IgG antibody to S. japonicum is stable, reliable and suitable for the screening of schistosomiasis in the field.