Epigenomics · 2018
Whole-methylome analysis of circulating monocytes in acute diabetic Charcot foot reveals differentially methylated genes involved in the formation of osteoclasts.
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Pasquier Jennifer, Spurgeon Mark, Bradic Martina, Thomas Binitha, Robay Amal, Chidiac Omar, Dib Marie-Joe, Turjoman Rebal, Liberska Alexandra, Staudt Michelle, Fakhro Khalid A, Menzies Robert, Jayyousi Amin, Zirie Mahmoud, Suwaidi Jassim Al, Malik Rayaz A, Talal Talal, Rafii Arash, Mezey Jason, Rodriguez-Flores Juan, Crystal Ronald G, Abi Khalil Charbel
Аннотация
AIM: To assess whether DNA methylation of monocytes play a role in the development of acute diabetic Charcot foot (CF).
PATIENTS & METHODS: We studied the whole methylome (WM) of circulating monocytes in 18 patients with Type 2 diabetes (T2D) and acute CF, 18 T2D patients with equivalent neuropathy and 18 T2D patients without neuropathy, using the enhanced reduced representation bisulfite sequencing technique.
RESULTS & CONCLUSION: WM analysis demonstrated that CF monocytes are differentially methylated compared with non-CF monocytes, in both CpG-site and gene-mapped analysis approaches. Among the methylated genes, several are involved in the migration process during monocyte differentiation into osteoclasts or are indirectly involved through the regulation of inflammatory pathways. Finally, we demonstrated an association between methylation and gene expression in cis- and trans-association.
PATIENTS & METHODS: We studied the whole methylome (WM) of circulating monocytes in 18 patients with Type 2 diabetes (T2D) and acute CF, 18 T2D patients with equivalent neuropathy and 18 T2D patients without neuropathy, using the enhanced reduced representation bisulfite sequencing technique.
RESULTS & CONCLUSION: WM analysis demonstrated that CF monocytes are differentially methylated compared with non-CF monocytes, in both CpG-site and gene-mapped analysis approaches. Among the methylated genes, several are involved in the migration process during monocyte differentiation into osteoclasts or are indirectly involved through the regulation of inflammatory pathways. Finally, we demonstrated an association between methylation and gene expression in cis- and trans-association.